Project No. 20-57-18012 “Investigation of the process of deposition of Langmuir-Blodgett nanobiofilms and their interaction with various types of acoustic waves in piezoelectric structures”
Head: Gorbachev I.A.
The purpose of the interdisciplinary Project, jointly with the Laboratory of Nanoscience and Nanotechnology of the Bulgarian University of Architecture, Construction and Geodesy, is to create and study new sensor coatings based on composite biofilms obtained by the Langmuir-Blodgett method for new generation biosensors. The project involves the study and search for new approaches to the creation of sensor coatings based on Langmuir-Blodgett films with biologically active enzymes to be used in the development of new generation biosensors.
Project objectives:
1. Formation and study of ordered layers of hybrid structures based on phospholipids and enzyme molecules (glucose oxidase and alcohol oxidase) obtained by the Langmuir-Blodgett method and their transfer to a piezoelectric substrate.
2. Study of the interaction of Langmuir-Blodgett biofilms with acoustic waves of various types in piezoelectric structures.
3. Formation and study of a prototype of an artificial cell membrane based on a mixed monolayer of phospholipid molecules and membrane fractions of Gluconobacter oxydans bacterial cells for multisensor coatings.
4. Study of the electrophysical and biochemical properties of the developed sensor coatings based on composite biofilms.
5. Mutual work of the Russian and Bulgarian teams to study the properties of the resulting LB films and analyze the possibility of their application to create a new generation of acoustoelectronic biosensors.
The practical significance of the results of the Project lies in the creation of enzyme electrodes for the purposes of molecular biosensors, electrochemical energy when converting chemical energy into electrical energy, as well as analytical sensor devices that are highly selective in biomedical diagnostics.
The developed sensor coatings will make it possible to create a new generation of biosensors using amperometric, potentiometric and acoustoelectronic technologies for the analytical express determination of metabolites, pollutants, and superecotoxicants.
As a result of the implementation of the first year of the project, the following results were obtained:
– A study was made of the influence of the composition and ratio of components in mixed monolayers of fatty acids (lipids) and phospholipids on the dynamics of the adsorption of enzyme molecules (glucose oxidase and aclogol oxidase) in the LB-film.
– A study was made of the influence of the characteristics of the aqueous subphase and the concentration of water-soluble enzymes in it on the process of immobilization of the Langmuir monolayer of phospholipid molecules.
– A study was made of the formation of mixed monolayers based on water-insoluble polymer and phospholipid molecules and water-soluble enzymes.
– The technology of forming a prototype of an artificial membrane based on a mixed monolayer of phospholipid molecules and membrane fractions of Gluconobacter oxydans bacterial cells was carried out.
– The technology of transferring Langmuir monolayers immobilized with enzyme molecules onto various types of solid substrates, including those made of piezoelectric materials, was developed.
– A study was made of the composition, structure and morphology of Langmuir-Blodgett biofilms created by the Russian team, transferred to solid substrates, using microscopy and spectroscopy methods.
– A study was made of the composition, structure and morphology of the Langmuir-Blodgett biofilms created by the Bulgarian team, transferred to solid substrates, using microscopy and spectroscopy methods.
The following publications were published in the first year:
- Gorbachev Ilya A. Smirnov Andrey V., Glukhovskoy Evgeny G., Kolesov Vladimir V., Ivanov George R., Kuznetsova Iren E. Morphology of Mixed Langmuir and Langmuir–Schaefer Monolayers with Covered CdSe/CdS/ZnS Quantum Dots and Arachidic Acid Langmuir 2021, 37 (48), P.14105-14113 DOI (CrossRef): 10.1021/acs.langmuir.1c02345
- Gorbachev I. A. Smirnov A. V., Shamsutdinova E. S., Kashin V. V., Yudin S. G., Anisimkin V. I., Kolesov V. V., Kuznetsova I. E. Studying the Structural and Piezoelectric Properties of PVDF Films Obtained Using Langmuir‒Blodgett Technology Bulletin of the Russian Academy of Sciences: Physics 2021, 85 (6), P.603-607 DOI (CrossRef): 10.3103/S1062873821060101
- И.А. Горбачев М.М. Серебров, А.В. Смирнов, В.В. Колесов Модернизация Установки Для Формирования Монослоев Методом Ленгмюра-Блоджетт Математические методы в технологии и технике, 2021, В.1, С.20-23
- Ivanov George R. Gorbachev Ilya A., Amova Anna Electrical Properties of Langmuir-Blodgett Nano Thin Biofilms from a Fluorescently Labelled Phospholipid for Biosensor Applications 2021 XXXI International Scientific Symposium Metrology and Metrology Assurance (MMA) 2021, P.1-4.
- И.А. Горбачев А.В. Смирнов , В.В. Колесов , И.Е. Кузнецова Формирование сенсорных покрытий на основе молекулярных пленок ленгмюра-блоджетт с иммобилизованными ферментами 2021 Сборник тезисов конференции VII Международная конференция «Супрамолекулярные системы на поверхности раздела» 2021, С.68-69
- Горбачев И.А., Смирнов А.В., Колесов В.В. Фотосенсоры на основе молекулярных пленок Ленгмюра — Блоджетт фталоцианина меди СВЧ техника и телекомуникационные технологии 2021 С.242-243